Apoptotic and Inhibitory Effects on Cell Proliferation of Hepatocellular Carcinoma HepG2 Cells by Methanol Leaf Extract of Costus speciosus
Figure 1
Inverted phase contrast microscopy showing methanolic extract of Costus speciosus leaves or sorafenib (positive control) induced changes of HepG2 cells. The control cells were well adhered, displaying the normal morphology of HepG2 cells. In contrast, majority of HepG2 cells treated with methanol extract at the concentration of 100 μg/mL or more became round and shrunken and could not be affixed to the walls and floating in the medium (×400 magnification). (a) 50 μg/mL methanol extract; (b) 100 μg/mL methanol extract; (c) 200 μg/mL methanol extract; (d) 50 μg/mL sorafenib; (e) 100 μg/mL sorafenib; (f) 200 μg/mL sorafenib; (g) control.
