rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway

<table>Effects of rhPDGF-BB on osteogenic differentiation of diabetic BMSCs. Cells were treated with or without rhPDGF-BB (10 ng/mL or 50 ng/mL) for the indicated periods. (a) Real-time PCR analysis of ALP, Runx2, and OCN mRNA expression. (b) Western blot analysis for the relative Runx2 protein levels. (c) ALP staining on days 7 and 14 (16x). (d) Quantitative results of ALP assay on days 7 and 14. (e) Alizarin red staining for mineralized nodules on day 21 (50x). (f) von Kossa staining for mineralized nodules on day 21 (50x). *<svg height="11.375" id="M8" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, versus control, and <sup>#</sup><svg height="11.375" id="M9" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, versus 10 ng/mL.</table>

BioMed Research International

fig2

Figure 2

Figure 2: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway 

Figure 2 | rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway 