rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway

Figure 3

rhPDGF-BB activated ERK pathway in diabetic BMSCs. Cells were pretreated with or without 20 μM PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB for the indicated periods. The unmanipulated cells were used as controls. (a) Representative image of western blotting of p-ERK, ERK, and β-actin (𝑛=3). (b) The ratio of p-ERK/ERK intensity were quantified by densitometry and expressed graphically. *𝑃<0.05, versus control, and ^#𝑃<0.05, versus 10 ng/mL.

(a)

(b)