Mas-Mediated Antioxidant Effects Restore the Functionality of Angiotensin Converting Enzyme 2-Angiotensin-(1–7)-Mas Axis in Diabetic Rat Carotid
Figure 4
levels in carotid endothelial cells from control or diabetic rats. Dot plots show the gates of carotid endothelial cells from nontreated control rats (a) or from nontreated (b) or angiotensin-(1–7)-treated (c) diabetic rats. Histograms show the fluorescence emitted by DHE-loaded carotid endothelial cells from nontreated control rats (d) or from nontreated (e) or angiotensin-(1–7)-treated diabetic rats (f). Effects of the in vitro pretreatment with tiron, apocynin, or losartan or the chronic (in vivo) treatment with angiotensin-(1–7) combined or not with A779 on the fluorescence emitted by DHE-loaded endothelial cells samples (g). The values are significantly different (; ) from the respective blank samples from nontreated rats (), from nonpretreated DHE-loaded endothelial cells samples from nontreated control () or diabetic () rats, or from the respective DHE-loaded endothelial cells samples from angiotensin-(1–7)-treated rats (#).
