Mas-Mediated Antioxidant Effects Restore the Functionality of Angiotensin Converting Enzyme 2-Angiotensin-(1–7)-Mas Axis in Diabetic Rat Carotid
Figure 5
H2O2 levels in carotid endothelial cells from control or diabetic rats. Dot plots show the gates of carotid endothelial cells from nontreated control rats (a) or from nontreated (b) or angiotensin-(1–7)-treated diabetic rats (c). Histograms show the fluorescence emitted by CDCF-DA-loaded carotid endothelial cells from nontreated control rats (d) or from nontreated (e) or angiotensin-(1–7)-treated diabetic rats (f). Effects of the in vitro pretreatment with PEG-catalase, tiron, apocynin, or losartan or the chronic (in vivo) treatment with angiotensin-(1–7) combined or not with A779 on the fluorescence emitted by CDCF-DA-loaded endothelial cells samples (g). The values are significantly different (; ) from the respective blank samples from nontreated rats (), from nonpretreated CDCF-DA-loaded endothelial cells samples from nontreated control () or diabetic () rats, or from CDCF-DA-loaded endothelial cells samples from angiotensin-(1–7)-treated diabetic rats (#).
