Dual Effects of Cigarette Smoke Extract on Proliferation of Endothelial Progenitor Cells and the Protective Effect of 5-aza-2′-deoxycytidine on EPCs against the Damage Caused by CSE
Figure 1
The morphological changes of endothelial progenitor cells (EPCs) during culture. (a) representative photomicrographs of EPCs on day 1 of the culture. The EPCs formed round, the sizes of cells were almost same, and the cells were suspended in the culture medium (arrow 1); (b) on day 4 of the culture, the cells were attached to each other, the sizes were getting enlarged, and the shapes became oval, spindle, or polygonal (arrow 2); (c) on day 7 of the culture, the cells shaped to fusiform or polygon patterns and contacted each other to attempt to form capillary structure (arrow 3). Scale bar represents 50 μm.
