Dual Effects of Cigarette Smoke Extract on Proliferation of Endothelial Progenitor Cells and the Protective Effect of 5-aza-2′-deoxycytidine on EPCs against the Damage Caused by CSE
Figure 2
The identification of EPCs by double positive staining with 1,1′-dioctadecyl-3,3,3′,3-tetramethylindocarbocyanine perchlorate (Dil) labeled acetylated low density lipoprotein (Dil-acLDL) and FITC labeled ulex europaeus agglutinin-1 (FITC-UEA-1). The laser scanning confocal microscope (LSCM) illustrated that the cells on day 7 of the culture displayed red cytoplasm when stained with Dil-acLDL (a), green cytomembrane when combined with FITC-UEA-1 (b), orange when double positively stained with Dil-acLDL and FITC-UEA-1) (c), and blue when stained with DAPI in nuclear localization (d). Scale bar represents 10 μm.
