Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification
Figure 2
The alignment of C-probe target specific termini that detect SNP targets via the RAM reaction. The figure shows Wt target strands (“Wt Str.”) and Mt C-probe target-specific terminal sequence regions in blue and the Mt target strands (“Mt Str.”) and Wt C-probe terminal target specific regions in red. Wt and Mt C-probe termini are shown as if bound to their respective targets, and joined by a target-non-homologous linker represented by a dotted line. The SNP base is shown in bold-face in the target strands and on the 3′ terminal base of each C-probe. Strand specific capture probes (not show) bind proximally downstream (3′) of the C-probe binding domain. (a) shows the FV and PT target/probe arrangement, WtC+, MtC−, and (b and c) the MtC+, WtC− format used for the detection of both MTHFR SNPs, MTHFR 677 C-T and MTHFR 1298 A-C respectively.
