Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification

Table 1

Nucleotide sequences of C-probes, capture probes and primers.

Oligo Name	Sequence	Targeted locus	Targeted allele	Targeted strand	Oligo role
Cpr8FVWt+	GCCTGTCCAGGGATCTGCTCTTACAATACGAGAACACCCGATTGAG; AGAGTTTGGAAGTGTAGGCGTGAAGTCCATAACACATACCTGTATTC; CTC	FV 1691 G-A	Wt	+	C-probe
CPFV+	Bio-TCAGAATTTCTGAAAGGTTACTTC-Spacer C3	FV 1691 G-A	Wt	+	Target capture
Cpr8Fwd61_22	ACACTTCCAAACTCTCTCAATC	FV 1691 G-A	Wt	+	RAM Fwd Primer
Cpr8Rvs03_20	CTGTCCAGGGATCTGCTCTT	FV 1691 G-A	Wt	+	RAM Rev Primer
Cpr9FVMt−	AGGAATACAGGTATTTTGTCCTTGAAGTAACCCTCGTGAAAGCCCTA; CTCTATGAAATCTTGTAGCAGGACTCCGTTTAGCAGCACTGGACAG; GCA	FV 1691 G-A	Mt	−	C-probe
CPFV−	Bio-CCTCTGGGCTAATAGGACTACTTCTAATCTG-Spacer C3	FV 1691 G-A	Mt	−	Target capture
Cpr9Fwd73_21	GAGTCCTGCTACAAGATTTCA	FV 1691 G-A	Mt	−	RAM Fwd Primer
Cpr9Rvs87_21	TGGACAGGCAAGGAATACAGG	FV 1691 G-A	Mt	−	RAM Rev Primer
Cpr10PTWt+	GCTGAGAGTCACTTTTATTGGGAACCATAGAACTTCGTATCGCTCGC; ACAAGAATCGTGTTTGAACAAGTAGGTGAATGGGCATTGAGGCTC	PT 20210 G-A	Wt	+	C-probe
CPPT+	Bio-CTGCCCATGAATAGCACTGG-Spacer C3	PT 20210 G-A	Wt	+	Target capture
Cpr10Fwd48_20	TGCGAGCGATACGAAGTTCT	PT 20210 G-A	Wt	+	RAM Fwd Primer
Cpr10Rvs4_24	GAGAGTCACTTTTATTGGGAACCA	PT 20210 G-A	Wt	+	RAM Rev Primer
Cpr12PTMt−	AGCCTCAATGCTCCCAGTGCTACGAGATAGACTGTGTCCTTCGGTG; CGATGTTACCTTATGTGAATCACGATAAACTTCATTTGACTCTCAGC; A	PT 20210 G-A	Mt	−	C-probe
CPPT−	Bio-CCGTGAAAGAATTATTTTTGTGTTTC-Spacer C3	PT 20210 G-A	Mt	−	Target capture
Cpr12Fwd57_20	AGGTAACATCGCACCGAAGG	PT 20210 G-A	Mt	−	RAM Fwd Primer
Cpr12Rev 5_20	TCAATGCTCCCAGTGCTACG	PT 20210 G-A	Mt	−	RAM Rev Primer
Cpr13MTH677Wt−	CGATTTCATCATCACGCAGCTTTTCTTTGGTTATTGAAAGGTTCATC; GCTTAGTGATACCCCGTCGCCAGCACTCGTCATCGCCTACCTGCGG; GAGC	MTHFR 677 C-T	Wt	−	C-probe
CPMTH677−	Bio-TTTGAGGCTGACCTGAAGCAC-Spacer C3	MTHFR 677 C-T	Wt	−	Target capture
Cpr13Fwd61_20	GGGTATCACTAAGCGATGAA	MTHFR 677 C-T	Wt	−	RAM Fwd Primer
Cpr13Rev16_20	GCAGCTTTTCTTTGGTTATT	MTHFR 677 C-T	Wt	−	RAM Rev Primer
Cpr14MTH677Mt+	CTCCCGCAGACACCTTCTCCTTCCACGACGGAGGTATTGACTCGGG; ATTTCCCAAAAGCAACAGATGGTGCCCCTATGATAGATGATGAAATC; GA	MTHFR 677 C-T	Mt	+	C-probe
CPMTH677+	Bio-GCGGAAGAATGTGTCAGCCTC-Spacer C3	MTHFR 677 C-T	Mt	+	Target capture
Cpr14Fwd 56_20	TTTGGGAAATCCCGAGTCAA	MTHFR 677 C-T	Mt	+	RAM Fwd Primer
Cpr14Rev 16_20	TCTCCTTCCACGACGGAGGT	MTHFR 677 C-T	Mt	+	RAM Rev Primer
Cpr15MTH1298Wt−	AAGTGTCTTTGAAGTCTTCGTTCTTTACCTCACCAAGTCATCGTCCG; CTCGTAGATAATGATAAGTGAGTAAAGTTATGAATGTTCTACTCCAG; TGAAGA	MTHFR 1298 A-C	Wt	−	C-probe
CPMTH1298−	Bio-GAGGAGCTGCTGAAGATGTGG-Spacer C3	MTHFR 1298 A-C	Wt	−	Target capture
Cpr15Fwd11_25	GAAGTCTTCGTTCTTTACCTCACCA	MTHFR 1298 A-C	Wt	−	RAM Fwd Primer
Cpr15Rev64_24	TTATCATTATCTACGAGCGGACGA	MTHFR 1298 A-C	Wt	−	RAM Rev Primer
Cpr16MTH1298Mt+	CTTCACTGGTCAGCTCCTCCCCTACAGCAAAGCGGCGTCTGTGCGA; AGATAATGATAAGTGAGTAAAGTTATGGATGGACGAAGAAAGACACT; TG	MTHFR 1298 A-C	Mt	+	C-probe
CPMTH1298+	Bio-ACCATTCCGGTTTGGTTCTCCC-Spacer C3	MTHFR 1298 A-C	Mt	+	Target capture
Cpr16Fwd 7_20	TGGTCAGCTCCTCCCCTACA	MTHFR 1298 A-C	Mt	+	RAM Fwd Primer
Cpr16Rev61_26	CACTTATCATTATCTTCGCACAGACG	MTHFR 1298 A-C	Mt	+	RAM Rev Primer