Research Article
Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification
Table 2
C-probe and primer concentrations used in hybridizations and RAMs respectively. Primer naming conventions are given in [
13].
| Allele | C-Probe | C-Probe Concentration in 2.5X buffer (nM) | Primer pair | Primer concentration (uM) in elution buffer |
| FV 1691 G-A Wt | Cpr8FVWt+ |
0.4 | Cpr8FVFwd61_22 | 2.5 | Cpr8Rvs03_20 | 2.2 | FV 1691 G-A Mut | Cpr9FVMt− |
0.25 | Cpr9Fwd73_21 | 2.5 | Cpr9Rvs87_21 | 1.5 | PT 20210 G-A Wt | Cpr10PTWt+ |
0.1 | Cpr10Fwd48_20 | 1.7 | Cpr10Rvs04_24 | 1 | PT 20210 G-A Mut | Cpr12PTMt− |
0.125 | Cpr12Fwd57_20 | 3 | Cpr12Rev05_20 | 1.8 | MTHFR 677 C-T Wt | Cpr13MTH677Wt− |
0.25 | Cpr13Fwd61_20 | 3.6 | Cpr13Rev16_20 | 2.2 | MTHFR 677 C-T Mut | CPMTH677Mt+ |
1.25 | Cpr14Fwd56_20 | 2.5 | Cpr14Rev 16_20 | 1.5 | MTHFR 1298 A-C Wt | Cpr15MTH1298Wt− |
0.125 | Cpr15Fwd11_25 | 1.2 | Cpr15Rev64_24 | 0.7 | MTHFR 1298 A-C Mut | Cpr16MTH1298Mt+ |
0.25 | Cpr16Fwd 7_20 | 1.4 | Cpr16Rev61_26 | 0.9 |
|
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All capture probes concentrations in the 2.5X buffer were 2.5 nM.
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