Detection of Genetic Variations in Coagulopathy-Related Genes Using Ramified Rolling Circle Amplification

Table 2

C-probe and primer concentrations used in hybridizations and RAMs respectively. Primer naming conventions are given in [13].

Allele	C-Probe	C-Probe Concentration in 2.5X buffer (nM)	Primer pair	Primer concentration (uM) in elution buffer
FV 1691 G-A Wt	Cpr8FVWt+	0.4	Cpr8FVFwd61_22	2.5
			Cpr8Rvs03_20	2.2
FV 1691 G-A Mut	Cpr9FVMt−	0.25	Cpr9Fwd73_21	2.5
			Cpr9Rvs87_21	1.5
PT 20210 G-A Wt	Cpr10PTWt+	0.1	Cpr10Fwd48_20	1.7
			Cpr10Rvs04_24	1
PT 20210 G-A Mut	Cpr12PTMt−	0.125	Cpr12Fwd57_20	3
			Cpr12Rev05_20	1.8
MTHFR 677 C-T Wt	Cpr13MTH677Wt−	0.25	Cpr13Fwd61_20	3.6
			Cpr13Rev16_20	2.2
MTHFR 677 C-T Mut	CPMTH677Mt+	1.25	Cpr14Fwd56_20	2.5
			Cpr14Rev 16_20	1.5
MTHFR 1298 A-C Wt	Cpr15MTH1298Wt−	0.125	Cpr15Fwd11_25	1.2
			Cpr15Rev64_24	0.7
MTHFR 1298 A-C Mut	Cpr16MTH1298Mt+	0.25	Cpr16Fwd 7_20	1.4
			Cpr16Rev61_26	0.9

All capture probes concentrations in the 2.5X buffer were 2.5 nM.