Effects of Growth Phase and Temperature on Activity within a Listeria monocytogenes Population: Evidence for RsbV-Independent Activation of at Refrigeration Temperatures
Figure 1
Levels of and OpuCA at selected points of growth at 37°C and 4°C. (a) levels were determined with Western blotting and rabbit polyclonal anti- antibodies in L. monocytogenes wild-type grown in BHI at 37°C at 60 min intervals from mid-exponential to stationary phase. OpuCA levels were monitored with Western blotting and chicken polyclonal anti-OpuCA antibodies in L. monocytogenes wild-type and ΔsigB grown in BHI at 37°C at 50 min intervals from mid-exponential to stationary phase. (b) Levels of OpuCA were determined in L. monocytogenes wild-type, ΔsigB, and ΔopuCA grown in BHI up to exponential phase (OD600 0.6) at 4°C in comparison to 37°C.
