Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs
Figure 1
Lung cells were transfected with various formulations of CO3Ap/pDNA, carrying constant amount of pDNA (2 μg). (a) Transfection efficiency of CO3Ap/pDNA complexes prepared in 1 mL DMEM supplemented with 10% serum protein. (b) Transfection efficiency of CO3Ap/pDNA complexes prepared in 1 mL serum-free DMEM. (c) Transfection efficiency of CO3Ap/pDNA complexes prepared in 100 μL serum-free DMEM. Luciferase expression was measured as RLU/mg protein at 48 hr after transfection. The error bar represents standard error of the mean (SEM) for all data sets. The result is considered to be statistically significant if values were <0.05. Data are presented as mean ± S.E.M of experiments conducted in triplicate.
