Gene Delivery Potential of Biofunctional Carbonate Apatite Nanoparticles in Lungs
Figure 3
Morphological appearance and size of CO3Ap/pDNA complexes. Formulated CO3Ap complexes using various amount of CaCl2 with 2 µg of pDNA were subjected to FESEM and Nanophox analyses. Complex formulation by (a) CO3Ap (6 μL)/pDNA shows much smaller aggregated particles when compared to (b) CO3Ap (8 μL)/pDNA with individual particle size of about 150 nm. Scale: (×100 000) (c) CO3Ap/pDNA size analysis. The complex size was found to be proportional to the increasing amount of CaCl2 used to prepare the CO3Ap/pDNA formulations. The error bar represents standard error of the mean (SEM) for all data sets. Data are presented as mean ± S.E.M of experiments conducted in triplicate.
