A Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Viral and Bacterial Pathogens of Infectious Diarrhea
Figure 1
Electrophoresis results of the PCR products on automatic electrophoresis. All of the targets were identified successfully, and no mispriming was observed in either tube. Lanes 1 to 6 in part (a): NoV GI (162 bp), RVA (210 bp), NoV GII (279 bp), HAstV (326 bp), EAds (388 bp), and HBoV (412 bp), respectively. Lane 7: PCR products of six premixed viral targets in tube 1 (Qiaxcel-V assay). Lanes 8 to 14 in part (b): Shigella (213 bp), Vibrio parahaemolyticus (246 bp), diarrheagenic Escherichia coli (293 bp), Salmonella (323 bp), Yersinia (389 bp), Vibrio cholera (507 bp), and Campylobacter jejuni (627 bp). Lane 15: PCR products of seven premixed bacterial targets in tube 2 (Qiaxcel-B assay).
