Development of a Stereotaxic Device for Low Impact Implantation of Neural Constructs or Pieces of Neural Tissues into the Mammalian Brain

Figure 3

Diagram of the implantation device attached to a stereotaxic frame. An implantation accessory bracket (4) with the stationary section (8) of the translation stage is fitted to the arm of the stereotactic frame (1). A movable section of the translation stage (2) allows for retracting of the syringe (3) by turning the micrometric screw (7). After insertion into the brain, using the stereotaxic frame, the needle (5) is retracted jointly with the syringe, while the plunger, being fixed to the accessory bracket (4) is stationary together with the supplementary plunger within the needle and the scaffold itself. As a result, scaffold (6) is displaced into a cavity formed by the retracting needle. Photograph (lower left) shows the implantation device mounted on the stereotactic frame.