A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
Figure 1
(a) Flowchart outlining the molecular diagnostic procedure: from viral RNA extraction to HA and NA fragments sequencing and analysis. (b) Diagram showing the related positions of specific primers and the three PCR amplicons (P1, P2, and F) during the traditional PCR amplification step. UTR: untranslated region. P1: partial 1 fragment (primers Uni12 F and Mid R). P2: partial 2 fragment (primers Mid F and Uni12 R). F: large fragment (primers Uni12 F and Uni12 R).
