A Complete Molecular Diagnostic Procedure for Applications in Surveillance and Subtyping of Avian Influenza Virus
Figure 4
Amplification and standard curves of two-step SYBR-Green qPCR for influenza virus detection and subtyping using reference H4N8 stain as model. (a) M gene, (b) NA gene, (c) HA gene; (i) cDNA template concentration in each amplification curve: (1) 5 pg/mL; (2) 0.5 pg/mL; (3) 0.05 pg/mL; (4) 5 fg/mL; (5) 0.5 fg/mL; (6) 0.05 fg/mL; (7) 5 ag/mL. (ii) Correlation coefficient and PCR efficiency for each gene were detailed in each standard curves’ chart.
