Enhanced Prevalence of Plasmatic Soluble MHC Class I Chain-Related Molecule in Vascular Pregnancy Diseases
Figure 3
Quantification of INF-γ secretion by the NK-92 NK cell line after 72 hours incubation in media containing 20% plasma from representative sMIC-positive vascular pregnancy diseases (VPD, sMIC+) and gestational age-matched sMIC-negative plasma normal pregnancies (NP, sMIC−). (a) ELISA detection of INF-γ levels in supernatants of NK-92 cells cultured in 20% plasma. (b) Real-time PCR transcriptional analysis of INF-γ mRNA levels in NK-92 cells. mRNA transcript levels are represented as relative copy numbers per 106 GAPDH transcripts. Results are expressed as median and 25–75 interquartile ranges ().
