The Role of Bioactive Lipids in Stem Cell Mobilization and Homing: Novel Therapeutics for Myocardial Ischemia
Figure 4
Effect of S1P on cardiac and endothelial protein expression in BMSPCs. Representative confocal and matrigel assay images of BMSPCs cultured for 4 and 2 weeks in culture medium supplemented with 250 nM of S1P. Panels (a)–(c) depict elongated cells indicative of cardiomyocyte morphology. (a) Visualization of early myocardial protein expression (GATA4 and Nkx-2.5—green). (b) Staining of late myocardial protein expression (myosin heavy chain and Troponin—red). (c) Overlay of (a) and (b), depicting colocalization of myocardial protein expression in the cytoplasm and cardiac transcription factors around the nucleus. Panel (d) depicts round cells indicative of endothelial morphology and visualization of endothelial protein expression (VWF, PDGFrα, and PDGFrβ—red). All cells were stained with DAPI to visualize the nuclei (blue). (e) and (f) matrigel assay images demonstrate the capillary-like structure formation by BMSPCs cultured in S1P medium.
