A Novel Phospholipase A2 (D49) from the Venom of the Crotalus oreganus abyssus (North American Grand Canyon Rattlesnake)
Figure 3
Phospholipase A2 activities of CoaPLA2 from Crotalus Oreganus abyssus. (a) Phospholipase A2 activities of CoaPLA2 and total venom of Crotalus Oreganus abyssus, Crotalus viridis viridis, and Crotalus viridis nuntius using a nonmicellar substrate (4-nitro-3-octanoyloxy benzoic acid); (b) Phospholipase A2 activities of CoaPLA2 and total venom of Crotalus Oreganus abyssus, Crotalus viridis viridis, and Crotalus viridis nuntius using a micellar substrate (1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphoglycerol); (c) influence of pH variations on the enzymatic activity of CoaPLA2; (d) influence of temperature variations on the enzymatic activity of CoaPLA2; (e) analysis of the influence of calcium ions and other divalent cations on the phospholipase A2 activity of CoaPLA2. Results are expressed as mean ± SD of three independent experiments performed in triplicate ().
