Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors
Figure 1
Protein expression of PlgK1-5 in vitro and in vivo. Cells were transfected with pMock or pK1-5 and incubated for 48 hours. Cells were harvested, and protein was isolated. Tumour and liver tissue fragments from mice treated with NaCl, DOTAP alone, pMock, or pK1-5 were homogenised using the Precellys system and protein was isolated. Whole protein content was determined and 50 g protein was subjected to SDS-PAGE. Following semidry blotting, PlgK1-5 was detected using an angiostatin-specific antibody. GAPDH was used as a control. Ctrl: NaCl or plain medium control; Dotap: DOTAP control; Mock: pMock; K1-5: pK1-5.
