Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors
Figure 3
VEGF expression after pK1-5 treatment in vitro. Cells were transfected and incubated for 48 hours. Cells and supernatants were harvested, and cells were solubilized in PBS/complete and lysed with repeated freeze-thaw cycles. Protein levels were analysed and VEGF levels were quantified with human (HuH7) or murine (Hepa1-6, Hepa129) VEGF ELISAs. VEGF levels in supernatants were correlated to OD acquired by MTT assays. (a) VEGF levels in cell lysates. Data are shown as mean pg VEGF/100 g protein ± SEM. (b) VEGF levels in supernatants, MTT correlated. Data are shown as mean pg VEGF/mL cell supernatant ± SEM. = 4, < 0.05 (compared to plain medium), < 0.05 (compared to pMock).
