Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors
Figure 5
STAT3 phosphorylation. 104 HuH7 (a), Hepa1-6 (b), or Hepa129 (c) were seeded on a sterile black 96-well tissue plate with clear bottom, transfected with Lipofectamine and pCMVTNT vectors, or treated with plain medium and incubated for 48 hours. STAT3 phosphorylation was quantified according to manufacturer’s protocol (STAT3 immunoassay, R&D systems). Fluorescence was measured with the GloMax Multi (Promega) ELISA reader. For STAT3, fluorescence was measured at 360 nm (excitation) and 450 nm (extinction) and at 540 nm (excitation) and 600 nm (extinction) for pSTAT3. Data are presented as relative fluorescence units ± SEM. = 4, < 0.05 (compared to plain medium), < 0.05 (compared to pMock).
