DNA Methylation Pattern in Overweight Women under an Energy-Restricted Diet Supplemented with Fish Oil

Figure 3

Methylation of the CpGs located in the studied regions of the genes (a) CD36, (b) FFAR3, (c) CD14, (d) PDK4, and (e) FADS1 in PBMC from control and fish oil-supplemented women during the 8-week weight loss treatment. Data represent mean ± SD (control 𝑛=5; fish oil 𝑛=7). DNA methylation was determined at baseline and endpoint samples by MassARRAY EpiTyper in triplicate. A general linear model with repeated measures was used to assess the impact of oral supplementation on DNA methylation (between subjects) across the weight loss treatment (within subjects). ^∗𝑃<0.05, differences due to weight loss (baseline versus endpoint); ^#𝑃<0.05, interaction between weight loss effect and oral supplementation.

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