DNA Methylation Pattern in Overweight Women under an Energy-Restricted Diet Supplemented with Fish Oil

Table 1

Primers used for the bisulfite conversion of DNA.

Symbol	Primers	Product size	CpG in region^a
CD36	L: GGGTTGAGAGTTTGTGTTTTATTTTT; R: TTCAAATACAATTACACTTTTTAAAATCAC	479	4
FFAR3	L: GGTAAATTGGATAAATGTTATTTTAGAGA; R: CAACAAAAAAACACCAAAATACTCC	385	9 (7)
CD14	L: GGAGGGAATTGAATGATATTTTAGG; R: AAATCTCCACCTCTACTACAAACACA	429	24 (20)
PDK4	L: TTTTGTTTTGAGTAAGGATTAATGA; R: TCCCAAACAAAAAAAATCACTAAAA	400	23
FADS1	L: TTGTAATTTTTAAGGGTTTTTAGGT; R: AAACAACTCACAACTAAACTACCAACA	419	37 (35)

aValues between parenthesis indicate the number of CpGs covered by Sequenom EpiTyper approach. L: left primer; R: right primer; Chr: chromosome; CD36: thrombospondin receptor; CD14: cluster of differentiation 14; FADS1: fatty acid desaturase 1; PDK4: pyruvate dehydrogenase kinase, isozyme 4; FFAR3: free fatty acid receptor 3.