Nuclear Distribution of RNA Polymerase II and mRNA Processing Machinery in Early Mammalian Embryos

Figure 1

Double immunolocalization of SC35 (a), (b) and hyperphosphorylated RNA polymerase II (PolII) (a′), (b′) in mouse embryos. Fluorescence of nuclei begins to be detected only at the early 2-cell stage (line a). Association of RNAP II with SC35 domains (speckles) is observed already at this stage and is increased when ZGA finishes (line b). Bar is 10 μm, according to [24], open access.