Rho/ROCK Signal Cascade Mediates Asymmetric Dimethylarginine-Induced Vascular Smooth Muscle Cells Migration and Phenotype Change
Figure 4
Rho/ROCK signal transduction pathway induced by ADMA leads to the activation of ERK signal pathway. ERK activity was determined after VSMCs were treated with or without ADMA for 15 min. The phosphorylation level of Tyr202/204-phosporylated ERK1/2 was detected. P-ERK1/2: phosphorous ERK1/2; ERK1/2: total ERK1/2. VSMCs pretreated by being transfected with pAV-CMV-GFP-DDAH2 or not, pretreated with or without Y27632 (10 μM) for 30 min, and pretreated with or without L-arginine (1 mM) for two hours were indicated at the bottom. The graphs represent the relative expression level of every group by comparing to wild type VSMCs without ADMA treatment. Data shown are the mean ± SEM from three independent experiments, ; .
