The Proteasome Inhibitor, MG132, Attenuates Diabetic Nephropathy by Inhibiting SnoN Degradation In Vivo and In Vitro
Figure 3
MG132 partially reversed high glucose-induced degradation of SnoN in vitro. rGMCs were treated with 30 mmol/L high glucose for 12 h, 24 h, and 48 h (a). Cells were treated with the indicated concentrations of glucose, mannitol, or MG132 for 48 h (b). SnoN expression after high glucose challenge for various times and various glucose concentrations was determined by Western blotting and RT-PCR. The gray graph shows the relative statistical values for SnoN protein and mRNA expression in each group. The data were normalized and are expressed as means ± SD. versus NC group; versus 30M group; the expressions of SnoN (c) and Arkadia (d) of rGMCs were detected by immunofluorescence and laser scanning confocal microscopy (630x). SnoN and Arkadia were detected in the cytoplasm as green fluorescence.
