Disruption of Lipid Rafts Interferes with the Interaction of Toxoplasma gondii with Macrophages and Epithelial Cells
Figure 7
Adhesion and internalization indices of T. gondii in LLC-MK2 cells (a) and murine macrophages (b) after treatment with cholera toxin-B (CTB) (0.1, 1, and 2 μg/mL) for 20 minutes at 4°C before the addition of parasites (50 : 1) at 37°C for 10 minutes. The cells were then fixed and stained. In LLC-MK2 cells, adhesion and internalization were reduced at all concentrations tested, and in macrophages, a significant reduction in internalization was observed at the higher concentrations. Parasite loads were quantified microscopically, and the data shown are the means ± SE of duplicated points from three independent experiments. . The results were normalized.
