On the Mechanism(s) of Membrane Permeability Transition in Liver Mitochondria of Lamprey, Lampetra fluviatilis L.: Insights from Cadmium

Figure 4

Action of Ca²⁺, P_i, laurate, and FCCP on the membrane permeability of winter lamprey liver mitochondria. A membrane permeabilization of isolated lamprey mitochondria was determined from their swelling (measured spectrophotometrically as apparent absorbance changes at 540 nm) in 250 mM sucrose medium (3 mM Tris-HCl, pH 7.3) at 20°C in the presence of succinate, rotenone, and oligomycin; mitochondrial protein content, 0.5 mg/mL. The concentrations used are: Ca²⁺, 20 𝜇M; P_i, 1 mM; FCCP, 100 nM; laurate, 30 𝜇M; CsA, 2 𝜇M. Experiments were repeated five times, with data presented being representative.