Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation
Figure 5
ENERGI-F704 attenuates LPS-induced nuclear translocation and production of NF-κB in BV2 cells. BV2 cells were stimulated with 200 ng/mL LPS and subsequently treated with ENERGI-F704 incorporating with or without compound C for 1 h. (a) After treatments, cells were fixed for immunocytochemical staining. NF-κB and nuclei were visualized using Alexa Fluor 488 (green) and DAPI (blue), respectively. (b) Ratio of nuclear: cytoplasmic immunofluorescence of NF-κB was assessed by microscopy image and quantified using ImageJ software. Ratio < 1 indicates brighter cytoplasmic staining for NF-κB, whereas ratios > 1 indicate brighter nuclear staining for NF-κB. (c) BV2 cells were incubated with 200 ng/mL LPS in the presence of ENERGI-F704 for 24 h. After treatments, cell lysates were used to determine the levels of NF-κB using Western blotting. Data are presented as the mean ± SEM of three independent experiments (one-way ANOVA; and , and ; specific comparison to LPS-treated control). Representative images of three independent experiments are shown.
