Newborn Screening for Sickle Cell Disease: Technical and Legal Aspects of a German Pilot Study with 38,220 Participants
Table 4
(a) Interrun precision of HPLC determined on two dried blood spot samples from newborns heterozygous for HbS. (b) Interrun precision of HPLC determined on a dried blood spot sample from a newborn heterozygous for HbC.
(a)
HbF mean %
CV %
HbA mean %
CV %
HbS mean %
CV %
Control 1
37.3
2.9
8.9
5.3
3.6
6.3
11
Control 2
40.1
4.8
1.9
27
1.0
50
8
(b)
HbF mean %
CV %
HbA mean %
CV %
HbC mean %
CV %
Control 3
39.6
5.7
1.7
22
1.3
28
11
Precision was reduced if certain hemoglobin fractions were present in an order close to the detection limit of 1% of the total AUC.
