Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells
Figure 7
Effects of silibinin on O-GlcNAc protein levels and TNF-α-induced activation of the NF-κB pathway. (a) ARPE-19 cells at 80% confluence were treated with 100 μM PUGNAc for 1 h and 25, 50, or 100 μM silibinin for 24 h. The blot was probed with an antibody against O-GlcNAc. The levels of protein expression were quantified by western blotting and are expressed as ratios relative to the average values for the control group. (b) The ARPE-19 cells were pretreated with 100 μM PUGNAc and/or 50 μM silibinin and were subsequently treated with or without TNF-α. A reporter gene assay was used to detect the activation of the NF-κB pathway. Asterisks () and () designate responses that are significantly different at and , respectively.
