The Mouse Median Nerve Experimental Model in Regenerative Research
Figure 3
Electron microscopy. (a)-(b) Representative electron micrographs of the control median nerve (a) and regenerated median nerve, 25 days after the injury (b), stained with uranyl acetate and lead citrate. The counting of the myelinated fibers is illustrated with yellow numbers. μm. (c) Shows the data obtained with the electron microscopic (EM) analysis. It shows the total number of myelinated fibers in control nerves and in regenerated nerves. (d) Comparison of stereological data from light microscopy (LM) and electron microscopy (EM). The figure illustrates that it is possible to count more myelinated axons with the electron microscope compared to the light microscope, in both control (Ctrl) and regenerated group (crush). Data are reported as means ± standard deviation of five samples in each group. Statistical analysis was performed using paired t-test (using SPSS 20.0 software). ; ; and .
