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BioMed Research International
Volume 2014 (2014), Article ID 704259, 9 pages
http://dx.doi.org/10.1155/2014/704259
Research Article

Intracerebroventricular Administration of Nerve Growth Factor Induces Gliogenesis in Sensory Ganglia, Dorsal Root, and within the Dorsal Root Entry Zone

1Department of Molecular Neurology, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), University Hospital Erlangen, Schwabachanlage 6, 91054 Erlangen, Germany
2Department of Neurosciences, University of California, La Jolla, San Diego, CA 92093-9157, USA
3Neurology Service, Veterans Affairs Medical Center, San Diego, CA 92161, USA

Received 25 August 2013; Revised 5 February 2014; Accepted 5 February 2014; Published 16 March 2014

Academic Editor: Gjumrakch Aliev

Copyright © 2014 Johannes C. M. Schlachetzki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Previous studies indicated that intracerebroventricular administration of nerve growth factor (NGF) leads to massive Schwann cell hyperplasia surrounding the medulla oblongata and spinal cord. This study was designed to characterize the proliferation of peripheral glial cells, that is, Schwann and satellite cells, in the trigeminal ganglia and dorsal root ganglia (DRG) of adult rats during two weeks of NGF infusion using bromodeoxyuridine (BrdU) to label dividing cells. The trigeminal ganglia as well as the cervical and lumbar DRG were analyzed. Along the entire neuraxis a small number of dividing cells were observed within these regions under physiological condition. NGF infusion has dramatically increased the generation of new cells in the neuronal soma and axonal compartments of sensory ganglia and along the dorsal root and the dorsal root entry zone. Quantification of BrdU positive cells within sensory ganglia revealed a 2.3- to 3-fold increase in glial cells compared to controls with a similar response to NGF for the different peripheral ganglia examined. Immunofluorescent labeling with S100β revealed that Schwann and satellite cells underwent mitosis after NGF administration. These data indicate that intracerebroventricular NGF infusion significantly induces gliogenesis in trigeminal ganglia and the spinal sensory ganglia and along the dorsal root entry zone as well as the dorsal root.