Toxic Damage Increases Angiogenesis and Metastasis in Fibrotic Livers via PECAM-1
Figure 7
Fibrosis development and metastasis formation. Mice were treated with ethanol and TAA. Every four weeks a subgroup of the animals were sacrificed and the livers explanted. 16 weeks after starting toxic damage, Hepa129 tumour cells were implanted. Ten days later, tumour bearing livers were explanted. Liver fragments were subjected to immunohistological staining. (a) PECAM-1 expression during fibrogenesis. Positive cells show a red-brownish colour. Blood vessels are indicated by a black arrow. (b) Exemplary macroscopic photographs of hepatomas in nonfibrotic and fibrotic livers. Metastases are indicated by white arrows. (c) Exemplary microscopic photographs of tumour bearing livers. Metastases adjacent to blood vessels (red brownish colour) are indicated by black arrows. Liver tissue is also stained red because of the peroxidase activity in the liver, which was not blocked in these samples.
