mAb CZP-315.D9: An Antirecombinant Cruzipain Monoclonal Antibody That Specifically Labels the Reservosomes of Trypanosoma cruzi Epimastigotes
Figure 1
Western blot analysis of antibodies against TcCruzipain. (a) Protein extracts of E. coli (Ec) and purified recombinant TcCruzipain (Czp) incubated with anti-TcCruzipain polyclonal serum (lane 1) or CZP-315.D9 monoclonal antibody (lane 2). (b) Total protein extracts of T. cruzi epimastigotes incubated with anti-TcCruzipain serum (lane 1), CZP-315 hybridoma supernatant (lane 2), CZP-315.D9 monoclonal antibody (lane 3) or preimmune serum (lane 4). Actin was used for normalization. (c) Protein extracts of vector-bacteria containing recombinant cruzipain domains incubated with polyclonal antibodies (serum) or with mAb CZP-315.D9 (mAb) against TcCruzipain. PP: prepro domain; Ca: catalytic domain; CT: C-terminus domain. The Bench Mark Protein Ladder was used to determine molecular weights. *Immature cruzipain; **mature cruzipain; ***TcActin.
