mAb CZP-315.D9: An Antirecombinant Cruzipain Monoclonal Antibody That Specifically Labels the Reservosomes of Trypanosoma cruzi Epimastigotes

Table 1

Characterization of mAb CZP-315.D9 with the SBA Clonotyping-HRP System. Numbers are the optical density (OD) values read at 450 nm. Only OD values above 0.300 were considered positive. RPMI medium was used as a negative control.

	Ig (H+L)	IgM	IgA	IgG1	IgG2a	IgG2b	IgG3	Kappa	Lambda
Anti-TcCruzipain serum	1.207	0.852	0.585	1.24	0.816	0.996	0.19	1.125	0.33
Hybridoma 315	0.99	0.085	0.077	1.259	0.512	0.078	0.069	0.362	0.058
mAb CZP-315.D9	0.788	0.055	0.056	1.105	0.085	0.164	0.065	0.459	0.133
Medium (RPMI)	0.059	0.056	0.049	0.064	0.068	0.061	0.072	0.108	0.059