Adrenergic Activation of Melatonin Secretion in Ovine Pineal Explants in Short-Term Superfusion Culture Occurs via Protein Synthesis Independent and Dependent Phenomena
Figure 4
Experiment IV. Effect of repeated adrenergic stimulation on melatonin secretion from the ovine pineal explants treated with transcription and translation inhibitors. The ovine pineal glands () were divided into four explants, which were randomly assigned to the experimental groups. The explants were incubated in the medium containing group I, 0.5% DMSO between 301 min and 720 min of the experiment; group II, 10 μM ACT D between 301 min and 720 min of the experiment; group III, 100 μM CLX between 301 min and 720 min of the experiment; and group IV, 100 μM ANS between 301 min and 720 min of the experiment. The explants from all groups were treated two times with 10 μM NE, from 360 min to 420 min and from 540 min and 600 min of the experiment. The mean melatonin secretion between 241 min and 300 min of the experiment was taken as 100%. The data presented are means. The values significantly different () between groups were signed with different letters. Blue bar is period of incubation in the medium with inhibitors; red bars are periods of incubation in the medium with NE.
