Inhibition of Hepatitis B Virus Replication by Helper Dependent Adenoviral Vectors Expressing Artificial Anti-HBV Pri-miRs from a Liver-Specific Promoter
Figure 2
Silencing of HBV replication in mice by HD Ads expressing anti-HBV pri-miR mimics from a CMV promoter. Mice were infected with a single dose of 5 × 109 infectious HD Ad particles expressing pri-miR-122/5, pri-miR-31/5, and pri-miR-31/5-8-9. Animals injected with saline or HD Ad Δ28 served as negative controls. (a) Time course of serum HBsAg concentrations, as measured by ELISA, following HD Ad administration. (b) Circulating viral particle equivalents (VPEs) were measured using qPCR following injection of HBV transgenic mice with HD Ads. Data are expressed as means with SEMs from each group of six mice. The statistical significance was calculated using a pair-wise comparison according to the Student -test. values less than 0.05 () or less than 0.01 () were considered statistically significant.
