Perinatal Hypoxia-Ischemia Reduces α7 Nicotinic Receptor Expression and Selective α7 Nicotinic Receptor Stimulation Suppresses Inflammation and Promotes Microglial Mox Phenotype
Figure 1
Decreased α7R expression 72 h after HI. Gene expression analysis of α7R, α4, and β2 receptor subunit levels in brains 24 and 72 h after hypoxia-ischemia (HI) in neonatal mice at age P9-10 ((a)–(c)). (a) At 24 h after HI there was no difference in gene expression of α7R; however, at 72 h α7R gene expression was significantly reduced in the injured (ipsilateral, Ipsi) hemisphere compared to the noninjured (contralateral, Contra) hemisphere. (b) Gene expression of α4R subunit was not influenced at 24 or at 72 h after HI. (c) β2R subunit expression was significantly increased in the injured (Ipsi) hemisphere compared to the noninjured (Contra) hemisphere at 24 h after HI. There was no difference in gene expression of β2R subunit at 72 h after HI. There was no difference between the sham animals (ipsilateral hemisphere) compared to the noninjured contralateral hemisphere in HI mice. Gene expression was normalized to YWHAZ and analyzed using CT method. Data are expressed as mean ± SEM, for contralateral versus ipsilateral hemisphere in HI mice, mice/group.
