Research Article
Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro
Table 7
Summary of purification of fibrinolytic enzyme from B. cereus IND1.
| | Procedure | Total activity (units) | Total protein (mg) | Specific (units/mg) | Purification (fold) | Yield (%) |
| | Crude enzyme | 27300 | 364 | 75 | 1 | 100 | | Ammonium sulfate fraction (30%–70% saturation) | 16505 | 128 | 129 | 1.72 | 60.5 | | DEAE cellulose | 5418 | 23 | 235 | 3.1 | 19.9 | | Sephadex G-75 | 3241 | 11.5 | 281 | 3.8 | 11.8 | | Casein-agarose | 1248 | 1.3 | 960 | 12.8 | 4.57 |
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