Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis

Figure 3

Analysis of FN-binding capability of EF-Tu through a LBA. The blot with the recombinant proteins was incubated with 20 μg/mL FN. Colorimetric detection of the bound bait protein was performed. We observed positive signal indicating the FN-binding capability of EF-Tu and the positive control Ag85 (red circles). AhpC was used as a negative control.