Detection Progress of Selected Drugs in TLC

Table 4

Characteristics of densitometric bands of stigmasterol after dipping and spraying with the use of particular visualizing reagents [47].

	10 µg of stigmasterol				5 µg of stigmasterol
	Area (AU)	Height (AU)	Angle α (°)	𝜆max (nm)	Area (AU)	Height (AU)	Angle α (°)	𝜆max (nm)
Without using visualizing reagent	7111	198	9	200	4400	126	9	200
Rhodamine B
Dipping	2264	86	32	200	1040	37.3	12	591
Spraying	4753	143.3	9	200	2721	90.6	14	200
Gentian violet
Dipping	2685	95.8	76	200	712	30.1	34	693
Spraying	5844	179.5	7.5	601	4655	137.7	8	602
Methylene violet
Dipping	3792	119	13.5	200	1177	58.2	12	200
Spraying	8988	251.2	7	200	6122	176.6	8	200
Methyl green
Dipping	3514	93.4	73	200	2237	55.7	39	200
Spraying	7857	216	7	200	5447	158.3	12	200
Malachite green
Dipping	2350	84.4	73.5	200	1052	37.3	37	200
Spraying	7740	221	10	638	4942	151.6	13	636
Janus blue
Dipping	2334	82.6	66	200	1429	47.5	36	663
Spraying	4024	147.3	9.5	674	2302	86.2	11.5	674