Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling

<table>UV absorption spectra of the patulin-HSA system. The concentration of HSA was 1.5 <i>μ</i>M, whereas the patulin concentrations were 0, 3.33, 6.67, 10.0, 13.3, 16.7, 20, 23.3, 26.7, and 30.0 <i>μ</i>M from a to j, respectively. Trisbuffer, pH = 7.4, and <svg height="11.6125" id="M103" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.45 11.6125" width="11.45" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.55)"><path d="M649 676l-22 -187l-33 -2q3 56 -12 94q-8 20 -31.5 26.5t-86.5 6.5h-74l-90 -491q-4 -23 -6 -36.5t1 -25t7 -16.5t18 -9t27 -5t41 -3l-6 -28h-286l4 28q68 5 84 18.5t28 76.5l94 491h-55q-74 0 -100.5 -6t-41.5 -23q-24 -29 -54 -98l-32 1q32 98 53 188h22q7 -18 15 -22
t37 -4h417q23 0 33.5 5t25.5 21h23z" id="x1D447"></path></g>
</svg> = 288 K.</table>

BioMed Research International

fig5

Figure 5

Figure 5: Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling 

Figure 5 | Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling 