Silencing of Plasma Membrane Ca2+-ATPase Isoforms 2 and 3 Impairs Energy Metabolism in Differentiating PC12 Cells
Figure 7
The effect of PMCAs downregulation on complexes of respiratory chain. (a) The activity of ETC complexes I–III was assessed spectrophotometrically at 37°C in mitochondrial fraction and is expressed as nmol/min after normalization to citrate synthase activity as described in Section 2. (b) Complex IV activity was determined in digitonin-permeabilized cells using an oxygraph (Anton Paar) according to the procedure described in Section 2 and is expressed as nmol O2/min after normalization to citrate synthase activity. The results are from independent determinations. versus control cells. : mock-transfected PC12, _2: PMCA2-reduced PC12, and _3: PMCA3-reduced PC12.
