Store-Operated Ca²⁺ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma

Figure 4

Store-operated Ca²⁺ entry is not sensitive to BTP-2 and Pyr 6 in mRCC cells. Biphasic Ca²⁺ response evoked by CPA ((a); 10 μM), thapsigargin (2 μM; (c)), or ionomycin (5 μM; (e)) in mRCC cells pretreated with BTP-2 (20 μM) for 20 min. Statistical analysis conducted on 80–90 cells per conditions revealed that BTP-2 did not affect the peak amplitudes of both Ca²⁺ release and Ca²⁺ entry stimulated by CPA (b), thapsigargin (d), or ionomycin (f). Similarly, 5 min preincubation with Pyr6 (10 μM) did not affect CPA-induced intracellular Ca²⁺ discharge and SOCE in mRCC cells (g). (h) Statistical evaluation of the effect exerted by Pyr6 on the peak amplitudes of both Ca²⁺ release and Ca²⁺ entry stimulated by CPA.

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