Genetic Diversity Analysis of Genotype 2 Porcine Reproductive and Respiratory Syndrome Viruses Emerging in Recent Years in China

Table 1

Primers used for amplification and sequencing of PRRSV genome and NSP2 HV region and ORF3 and ORF5 genes.

Name	Primer sequence	Location*	Length (bp)
5′-Outer-R	TTTCACTTCATCCCCACCAC	800	819
5′-Inner-R	CCCCGTTCATAAACTTGTAGAG	542	563
W1F	ATGACGTATAGGTGTTGGCTCT	1
W1R	TACTCTTTCAGGAAGGGTGG	1575	1575
W2F	ACGCTCTGGTGCGACTACTA	1362
W2R	AGGTTGTTCGGTTGTCTGATT	2253	892
W3F	CCTCCGTGGCGCAACAAGTCTTG	2115
W3R	CGATGATGGCTTGAGCTGAGTAT	3178	1064
W4F	TGAGCCTCTGGATTTGTCTGC	2949
W4R	GGCGATCTCATTAGGAGCAGTT	4329	1381
W5F	TGCTTAGGCTTGGCATTGTTG	4214
W5R	ACGGTGTTCAGTGAGGGCTTT	5564	1351
W6F	ACTAACATTGCTGGTCTCGTCA	5350
W6R	AAGGAAATCCAAGTCCTCGTC	6750	1401
W7F	TTGTGACCTCGCCAGTCCCAGTG	6500
W7R	CCAAAGCGTGCCATCAATCCC	7922	1423
W8F	GGTTGATGGTGGTGTTGTGCT	7787
W8R	GTCTTCTTTGGGTCCGTCTGG	9226	1440
W9F	TGGTCACCCTCATGGCCTTCT	9038
W9R	CAAATACATAGCAATGGGAGTCAAA	10323	1286
W10F	TTCCTGGATGAAGCGGCGTAT	10194
W10R	AACTCGGATGTATGAGGCGTAG	11573	1380
W11F	GGTGCTGGAAAGTGATGTTGG	11440
W11R	AAAGCGGGCATACCGTGTAAT	12774	1335
W12F	AGTGGTTTGGATGTGGTGGCT	12400
W12R	TGTTGTTGTTGCTGGCGTTGA	13803	1404
W13F	ATGTGCGACTGCTTCATTTCA	13599
W13R	TTTGCTGCTTGCCGTTGTTAT	14826	1228
W14F	TCCACTACGGTCAACGGCACAT	14702
W14R	GGATCCGGTACCTCTAGATCAGA		637
3′race adaptor-T	GGATCCGGTACCTCTAGATCAGATTTTTTTTTTTTTTTTTT
Nsp2-F	CCTCCGTGGTGCAACAAATCTTG	2115
Nsp2-R	CGATGATGGCTTGAGCTGAGTAT	3178	1064/1154
ORF3-F	CAGGGTCAAATGTAACCATAGTG	12506
ORF3-R	GGCAAGAAGAAAGCATGAGGAG	13457	952
ORF5-F	AGCCTGTCTTTTTGCCATTCT	13654
ORF5-R	CTTTTGTGGAGCCGTGCTATC	14335	682
ORF7F	TGATAACCACGCATTTGTCG	14668
ORF7R	GCCATTCACCACACATTCTTC	15228	561

∗The location is according to the genomic sequences of JXwn06 (GenBank Accession number: EF641008).