Combinatorial Control of Transgene Expression by Hypoxia-Responsive Promoter and MicroRNA Regulation for Neural Stem Cell-Based Cancer Therapy
Figure 4
Combinatorial effect of optHRP and miR-199a-5p on transgene regulation in NSC. (a) Schematic representation of the combinatorial expression cassettes containing the optHRP promoter and miRNA target sequences. optHRP, an artificially optimized hypoxia-responsive promoter; luc, luciferase reporter gene; miRNA target sequences as detailed in Table 1 were inserted into the 3′UTR; pA, polyA signal. (b) Transgene expression levels of different expression cassettes within NSCs under normoxic and hypoxic conditions are quantified by luciferase assays. Abbreviation: RLU, relative luminescence unit. Error bars: s.d. , .
